In Photoshop, whatever is transparent in the document will be transparent in a GIF or PNG image.

Simply use transparency in your layers within Photoshop and when you Save For Web as a GIF or PNG, that transparency is seen and used. There are no special steps you need to take to ensure transparency transfers to the final image.

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: Animating in Photoshop with transparency How to make a "Graphics Interchange Format" as Transparent in Photoshop or any other tool ?

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