: This really depends on what the image is you are working with. If its truly a black line on a white background @J0hj0h's suggestion of multiply is the best solution. It will turn the white

@Pierce403

This really depends on what the image is you are working with. If its truly a black line on a white background @J0hj0h 's suggestion of multiply is the best solution. It will turn the white part of your image into a perfect alpha channel.

If you are in a more complex scenario where the line may be red and the background blue, you can use color range to select the background color and turn it into an alpha channel.
helpx.adobe.com/photoshop/using/selecting-color-range-image.html
Select the background color, adjust for clarity, then hit mask on the bottom of the layers palette. Now your line should look good on any background. (works for human hair in photos ect as well.)

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